THE USE OF A CHROMATOGRAPHIC COLUMN PACKED WITH AMIDE SORBENT FOR THE ANALYSIS OF PARABENS
https://doi.org/10.30895/1991-2919-2018-8-1-56-60
Abstract
The article investigates the applicability of the amino column XBridge Amide 150×4.6 mm for separation of 4-hydroxybenzoic acid esters (parabens) — based on experience with hydrophilic interaction liquid chromatography used for analysis of various compounds. The study analysed the retention times, elution order and resolution between the peaks of parabens and impurity A (4-hydroxybenzoic acid) while using acetonitrile : phosphate buffer mobile phases containing 7–10 % of acetonitrile and acetonitrile : water mobile phases containing 96–99 % of acetonitrile, and the above-mentioned column. It was demonstrated that reversed-phased chromatography was preferable for separation of paraben homologues, while hydrophilic interaction chromatography was preferable for analysis of 4-hydroxybenzoic acid impurity. When analysing potential mechanisms of retention of compounds by the XBridge Amide column it was suggested that, unlike amino groups, amide groups of the sorbent do not have affinity for phenolic hydroxyl groups of parabens in hydrophilic interaction liquid chromatography.
About the Authors
A. S. Osipov
Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Alexey S. Osipov - Chief Expert of the Laboratory of ChemicalPharmaceutical Preparations No. 2 of the Testing Centre for Evaluation of Medicinal Products’ Quality, Candidate of Biological Sciences.
8/2 Petrovsky boulevard, Moscow 127051O. A. Popova
Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Olga A. Popova - Head of the Laboratory of Chemical-Pharmaceutical Preparations No. 2 of the Testing Centre for Evaluation of Medicinal Products’ Quality.
8/2 Petrovsky boulevard, Moscow 127051
S. E. Milkina
Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Svetlana E. Milkina - Chief Expert of the Laboratory of ChemicalPharmaceutical Preparations No. 2 of the Testing Centre for Evaluation of Medicinal Products’ Quality.
8/2 Petrovsky boulevard, Moscow 127051
T. N. Gretskaya
Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Tatiana N. Gretskaya - Chief Expert of the Laboratory of ChemicalPharmaceutical Preparations No. 2 of the Testing Centre for Evaluation of Medicinal Products’ Quality, Candidate of Pharmaceutical Sciences.
8/2 Petrovsky boulevard, Moscow 127051
A. I. Sulimenkova
Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Aleksandra I. Sulimenkova - Leading Expert of the Laboratory of ChemicalPharmaceutical Preparations No. 2 of the Testing Centre for Evaluation of Medicinal Products’ Quality.
8/2 Petrovsky boulevard, Moscow 127051
References
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Review
For citations:
Osipov A.S., Popova O.A., Milkina S.E., Gretskaya T.N., Sulimenkova A.I. THE USE OF A CHROMATOGRAPHIC COLUMN PACKED WITH AMIDE SORBENT FOR THE ANALYSIS OF PARABENS. The Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products. 2018;8(1):56-60. (In Russ.) https://doi.org/10.30895/1991-2919-2018-8-1-56-60
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