Chromatographic column with nitrile sorbent USED in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography
Abstract
The possibility of using chromatographic columns Zorbax SB CN 150´4,6 mm (5 μm) for separation of hydroxycarbamide (hydroxyurea) and its impurity urea has been investigated. The mixture of acetonitrile and water has been used as a mobile phase used. Separation of analytes on nitrile columns under the conditions of hydrophilic interaction liquid chromatography is possible only when the content of acetonitrile in the mobile phase is more than 90 %. When performing chromatographic procedure with Zorbax SB CN on nitrile column, the change in elution order of hydroxycarbamide and urea has been detected, compared to the elution order on amino column Zorbax NH2.
Keywords
высокоэффективная жидкостная хроматография,
хроматография гидрофильных взаимодействий,
гидроксикарбамид (гидроксимочевина),
нитрильный сорбент,
фармакопея,
high-performance liquid chromatography,
hydrophilic interaction liquid chromatography,
hydroxycarbamide (hydroxyurea),
nitrile sorbent,
pharmacopoeia
About the Authors
A. S. Osipov
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation
Russian Federation
E. B. Nechaeva
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation
Russian Federation
L. A. Truhacheva
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation
Russian Federation
References
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2. European Pharmacopoeia. Edition 8.0. Monograph : Hydroxycarbamide.
3. The United States Pharmacopoeia 38. Monograph: Hydroxyurea.
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7. Osipov AS, Nechaeva EB. The use of chromatographic columns with nitrile and phenyl sorbents for the analysis of coordination compounds of platinum. Himiko- farmatsevticheskiy zhurnal 2014; 48(8): 45–8 (in Russian).
8. Osipov AS, Nechaeva EB, Mironova MM, Kovaleva EL. The use of liquid chromatography of hydrophilic interactions for separation of butylhydroxyanisole isomers. Himiko- farmatsevticheskiy zhurnal 2015; 49(3): 50–2 (in Russian).
Review
For citations:
Osipov A.S., Nechaeva E.B., Truhacheva L.A. Chromatographic column with nitrile sorbent USED in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography. The Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products. 2016;(3):58-61. (In Russ.)
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