Spectrophotometric Determination of Hydroxycinnamic Acids in Herbal Drugs. Part 2. Determination of Hydroxycinnamic Acids in the Presence of Polyphenolic Compounds (Review)

INTRODUCTION. Polyphenolic compounds of various classes have pronounced absorption bands overlapping in almost the same ultraviolet region. This superposition of bands complicates the quantification of total hydroxycinnamic acids in herbal drugs by direct spectrophotometry without additional sample preparation.

AIM. This study aimed to establish the possibility of quantifying hydroxycinnamic acids in herbal drugs by spectrophotometry in the presence of other polyphenolic compounds.

DISCUSSION.  This  review  analyses  publications   on   analytical   procedures   for the spectrophotometric quantification of hydroxycinnamic acids that are based on the preliminary separation or chemical modification of polyphenolic compounds (combining the use of spectrophotometry, chromatography, extraction, Arnow’s reagent, etc.). The described sample preparation can help to resolve absorption bands in the spectra of polyphenolic compounds. The results of assessing the characteristic parameters of these analytical procedures (selectivity, sensitivity, labour intensity, etc.) can serve as a basis for searching novel approaches to the quantitative determination of hydroxycinnamic acids in plants.

CONCLUSIONS. To determine the content of hydroxycinnamic acids in mixtures of polyphenolic compounds, analysts may use special sample preparation (combining spectrophotometry with chromatography and extraction) or chemical modification of the initial compounds. The acquired spectra could benefit from specific mathematical processing (derivative photometry and Vierordt’s method). However, even with the abovementioned approaches, it may not always be possible to increase the selectivity of an analytical procedure. Therefore, there is a need to further improve existing analytical procedures for the quantification of hydroxycinnamic acids and develop new ones.

1. Denisenko TA, Vishnikin AB, Tsyganok LP. Spectrophotometric determination of sum of phenolic compounds in plant using aluminum chloride, 18-molybdodiphosphate and Folin–Ciocalteu reagents. Analytics and Control. 2015;19(4):373–80 (In Russ.). https://doi.org/10.15826/analitika.2015.19.4.012

2. Kruglov DS, Agapkina AS, Svechnikova OP, Klochkova SA. Spectrophotometric determination of the amount of phenolic carboxylic acids of some plants of the Boraginaceae family. In.: Development, research and marketing of new pharmaceutical products. Collection of scientific papers. Pyatigorsk; 2007. V. 62. P. 73–5 (In Russ.).

3. Korotaeva MS, Fursa NS. Seasonal dynamics of the accumulation of phenolic compounds in the shoots of the marsh beetle. In.: Development, research and marketing of new pharmaceutical products. Collection of scientific papers. Pyatigorsk; 2007. V. 62. P. 71–3 (In Russ.).

4. Kim NE, Kim NO, Khanina MA, Nekrasova MF. The study of biologically active substances in Pyrola family plants: one-sided wintergreen (Orthilia secunda (L.) House) and round-leaved wintergreen (Pyrola rotundifolia L.) collected in various regions of Siberia. In.: Development, research and marketing of new pharmaceutical products. Collection of scientific papers. Pyatigorsk; 2007. V. 62. P. 65–7 (In Russ.).

5. Kruglov DS, Ilyinykh AV. Research of some compounds of phenolic nature in vegetative and generative organs of blueberries (Vaccinium myrtillus L.). In.: Development, research and marketing of new pharmaceutical product. Collection of scientific papers. Pyatigorsk; 2007. V. 62. P. 75–7 (In Russ.).

6. Krupennikova VG, Fedoseeva GM. Phenolcarbon acids of Scabiosa comosa and Scabiosa ochroleuca. Siberian Medical Journal. 2007;71(4):90–2 (In Russ.). EDN: PFBJJF

7. Chistyakova AS, Maltseva AA, Slivkin AI. Determination of the content of the sum of oxycoric acids in the grass of Jacob’s ladder. In.: Development, research and marketing of new pharmaceutical products. Collection of scientific papers. Pyatigorsk; 2011. V. 66. P. 216–7 (In Russ.).

8. Vdovenko-Martynova NN, Stepanyuk SN. Phenolic compounds of urban gravilate (Geum urbanum L.) grass. In.: Development, research and marketing of new pharmaceutical products. Collection of scientific papers. Pyatigorsk; 2007. V. 62. P. 27–8 (In Russ.).

9. Ovchinnikova SYa, Mezenova TD, Orlovskaya TV. Determination method chlorogenic acid planar chromatography. Modern Problems of Science and Education. 2013;(6):1022 (In Russ.). EDN: RVDCXN

10. Kompantseva EV, Ayrapetova AYu, Saushkina AS. Spectrophotometric determination of hydroxycinnamic acids in herbal drugs. Part 1. Direct spectrophotometry. Bulletin of the Scientific Centre for Expert Evaluation of Medical Products. Regulatory Research and Medicine Evaluation. 2024;14(2):181–95 (In Russ.). https://doi.org/10.30895/1991-2919-2024-14-2-181-195

11. Kosman VM, Zenkevich IG. Quantitative extraction-spectrophotometric determination of the total content of hydroxycinnamic acids in the presence of flavonoids in extractive substances of some medicinal plants. Plant Resources. 2001;37(4):123–9 (In Russ.). EDN: KBHJMK

12. Larkina MS, Kadyrova TV, Ermilova EV. Study of the dynamics of accumulation of phenol-carboxylic acids in the aboveground part of cornflower. Chemistry of Plant Raw Materials. 2008;(3):71–4 (In Russ.). EDN: JUVCWP

13. Sannikova EG, Popova OI, Kompantseva EV, Frolova OO. Study of phenolcarbonic acids of Salix triandra L. tendrils growing in the North Caucasus. Pharmacy and Pharmacology. 2015;(2):13–7 (In Russ.). EDN: UBRBNR

14. Khiteva OO. Determination of phenolic acids in the raw materials of willow species growing in the North Caucasus. In.: Development, research and marketing of new pharmaceutical products. Collection of scientific papers. Pyatigorsk; 2012. V. 67. P. 131–3 (In Russ.).

15. Karpova EA, Karakulov AV. Phenolic compounds of closely related species of the genus Rhododendron L. (Ericaceae). Turczaninowia. 2011;14(3):145–9 (In Russ.). EDN: OJJSTZ

16. Sampiev AM, Davitavyan NA. Assay of flavonoids, isoflavonoids, and phenolcarboxylic acids in the field restharrow. Pharm Chem J. 2009;43(7):399–405. https://doi.org/10.1007/s11094-009-0314-z

17. Sampiev AM, Nikiforova EV, Khochava MR. Corn silk: from revealing working substances before creation of technology economical processings of raw material. The message 1. A problem of normalization of quality and reception of preparations from vegetative raw material, working substances focused on the aintenance (contents). Phytochemistry corn silk as the first stage in an establishment of working substances. Kuban Scientific Medical Bulletin. 2006;(12):106–10 (In Russ.). EDN: HVYUDJ

18. Dvornikova LG, Turetskova VF. Study of phenolic compounds of corn silk, gathered in Altai Region. Chemistry of Plant Raw Materials. 2013;(2):127–34 (In Russ.). https://doi.org/10.14258/jcprm.1302127

19. Bubenchikova VN, Starchak UA. Development and validation of methods for quantitative determination hydroxycinnamic acid’s amount in the plant of Thymus L. genus. Problems of Biological, Medical and Pharmaceutical Chemistry. 2015;(5):14–8 (In Russ.). EDN: RULXHA

20. Lavshuk VV. Water-organic extraction of hydroxycinnamic acids from dandelion medicinal roots. In: Actual problems of modern medicine and pharmacy. Collection of abstracts of the International Scientific and Practical Conference of students and young scientists. Minsk; 2019. P. 1538 (In Russ.).

21. Lavshuk VV, Lukashov RI. Influence of ultrasound on the efficiency of extraction of hydroxycinnamic acids from a dandelion roots. Bulletin of the Bashkir State Medical University. 2019;(4):199–203 (In Russ.). EDN: HPLDKK

22. Matveeva VI, Ruchkina AG, Kobrakov KI, Shpigun LC, Shmakova NS. Comparative characteristics of some plants family heather extracts and the effectiveness of methods for their production. Chemistry of Plant Raw Materials. 2022;(2):105–12 (In Russ.). https://doi.org/10.14258/jcprm.20220210516

23. Terletskaya VA, Lukashov RI. Comparative analysis of the biologically active substances content in the herb extracts from white dead-nettle and spotted dead-nettle. In: Modern trends in the development of health-saving technologies. Collection of scientific papers of X International Scientific and Practical Conference. Moscow: VILAR; 2022. P. 290– 5 (In Russ.). https://doi.org/10.52101/9785870191058_290

24. Turovets AV, Lukashov RI. Quantitative determination of hydroxycoric acids and phenolic compounds in plants of the thistle genus. In: Modern trends in the development of health-saving technologies. Collection of scientific papers of X International Scientific and Practical Conference. Moscow: VILAR; 2022. P. 264–8 (In Russ.). https://doi.org/10.52101/9785870191058_264

25. Kostikova VA, Veklich TN. Content of phenolcarboxylic acids and flavonoids inleaves and inflorescences of Spiraea salicifolia L. (Rosaceae). In: Problems of studying the vegetation cover of Siberia. Proceedings of the VII International Scientific Conference, dedicated to the 135th anniversary of P.N. Krylov Herbarium of Tomsk State University and the 170th anniversary of the birth of P.N.Krylov. Tomsk: TSU; 2020. P. 64–6 (In Russ.). https://doi.org/10.17223/978-5-94621-927-3-2020-20.