Test-system for the assessment of GSK-3 inhibitors activity as antihypoxants and endothelial differentiation stimulators in vitro
Abstract
The present article describes the stages of the development of the test-system that allows to research the potency of GSK-3 inhibitors to induce endothelial progenitor differentiation and to increase cell survival in hypoxia in vitro. β4-thymosin, which shows anti-ischemic and angiogenic activity, has been used a reference preparation. The proposed experimental cell model can be useful in the preliminary testing of new chemical compounds in terms of their ability to treat ischemic disorders and it can be used as an alternative for procedures involving animal testing.
About the Authors
Yu. E. Burda
Belgorod State National Research University
Russian Federation
Russian Federation
S. V. Nadezhdin
Belgorod State National Research University
Russian Federation
Russian Federation
E. V. Zubareva
Belgorod State National Research University
Russian Federation
Russian Federation
M. V. Pokrovsky
Belgorod State National Research University
Russian Federation
Russian Federation
S. Yu. Burda
Kursk State Medical University
Russian Federation
Russian Federation
M. S. Shirina
Belgorod State National Research University
Russian Federation
Russian Federation
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Review
For citations:
Burda Yu.E., Nadezhdin S.V., Zubareva E.V., Pokrovsky M.V., Burda S.Yu., Shirina M.S. Test-system for the assessment of GSK-3 inhibitors activity as antihypoxants and endothelial differentiation stimulators in vitro. The Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products. 2016;(1):29-32. (In Russ.)
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