vedomostiregmedРегуляторные исследования и экспертиза лекарственных средствRegulatory Research and Medicine Evaluation3034-30623034-3453Federal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’)10.30895/1991-2919-2023-13-4-567-577vedomostiregmed-567Research ArticleИНСТРУМЕНТАЛЬНЫЕ МЕТОДЫINSTRUMENTAL METHODSСовременные методы идентификации и количественного определения сердечных гликозидовModern methods for identification and quantification of cardiac glycosideshttps://orcid.org/0000-0003-2191-1033ЕвдокимоваО. В.EvdokimovaO. V.

Евдокимова Ольга Владимировна, доктор фарм. наук, доцент

Петровский б-р, д. 8, стр. 2, Москва, 127051, Российская Федерация

Olga V. Evdokimova, Dr. Sci. (Pharm.), Associate Professor

8/2 Petrovsky Blvd, Moscow 127051, Russian Federation

evdokimovaov@expmed.ruhttps://orcid.org/0000-0001-6975-516XБекетоваА. В.BeketovaA. V.

Бекетова Анастасия Викторовна, канд. фарм. наук

Петровский б-р, д. 8, стр. 2, Москва, 127051, Российская Федерация

Anastasia V. Beketova, Сand. Sci. (Pharm.)

8/2 Petrovsky Blvd, Moscow 127051, Russian Federation

beketova@expmed.ruhttps://orcid.org/0000-0003-1289-4307НаумоваО. А.NaumovaO. A.

Наумова Ольга Анатольевна, канд. фарм. наук

Петровский б-р, д. 8, стр. 2, Москва, 127051, Российская Федерация

Olga A. Naumova, Сand. Sci. (Pharm.)

8/2 Petrovsky Blvd, Moscow 127051, Russian Federation

naumovaoa@expmed.ruhttps://orcid.org/0009-0001-9627-1395КлинковаИ. В.KlinkovaI. V.

Клинкова Ирина Васильевна

Петровский б-р, д. 8, стр. 2, Москва, 127051, Российская Федерация

Irina V. Klinkova

8/2 Petrovsky Blvd, Moscow 127051, Russian Federation

klinkovaiv@expmed.ruhttps://orcid.org/0000-0003-3720-9687ШемерянкинаТ. Б.ShemeryankinaT. B.

Шемерянкина Татьяна Борисовна, канд. фарм. наук

Петровский б-р, д. 8, стр. 2, Москва, 127051, Российская Федерация

Tatiana B. Shemeryankina, Cand. Sci.  (Pharm.)

8/2 Petrovsky Blvd, Moscow 127051, Russian Federation

shemeryankina@expmed.ruhttps://orcid.org/0000-0003-4551-8252ЛадыгинаЛ. А.LadyginaL. A.

Ладыгина Лиана Александровна

Петровский б-р, д. 8, стр. 2, Москва, 127051, Российская Федерация

Liana A. Ladygina

8/2 Petrovsky Blvd, Moscow 127051, Russian Federation

ladygina@expmed.ruhttps://orcid.org/0009-0006-2928-9858БущикК. С.BushchikK. S.

Бущик Кристина Сергеевна

Петровский б-р, д. 8, стр. 2, Москва, 127051, Российская Федерация

Kristina S. Bushchik

8/2 Petrovsky Blvd, Moscow 127051, Russian Federation

bushchikks@expmed.ruФедеральное государственное бюджетное учреждение «Научный центр экспертизы средств медицинского применения» Министерства здравоохранения Российской ФедерацииРоссияScientific Centre for Expert Evaluation of Medicinal ProductsRussian Federation202321112023134567577Copyright © Евдокимова О.В., Бекетова А.В., Наумова О.А., Клинкова И.В., Шемерянкина Т.Б., Ладыгина Л.А., Бущик К.С., 20232023Евдокимова О.В., Бекетова А.В., Наумова О.А., Клинкова И.В., Шемерянкина Т.Б., Ладыгина Л.А., Бущик К.С.Evdokimova O.V., Beketova A.V., Naumova O.A., Klinkova I.V., Shemeryankina T.B., Ladygina L.A., Bushchik K.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vedomostincesmp.ru/jour/article/view/567Актуальность

Актуальность. Сердечные гликозиды более двух столетий находят применение в медицине. Данные современных исследований позволяют рассматривать перспективу применения биологически активных веществ этой группы не только в кардиологии, но и для лечения вирусных, онкологических и других заболеваний. Таким образом, актуальность выбора методов контроля качества сердечных гликозидов возрастает.

Цель

Цель. На основе анализа отечественных и зарубежных стандартов качества, а также современных научных данных выявить перспективные методы идентификации и количественного определения сердечных гликозидов в лекарственном растительном сырье (ЛРС) и лекарственных препаратах и оценить возможность использования физико-химических методов взамен биологических.

Обсуждение

Обсуждение. Используемые в настоящее время методы стандартизации сердечных гликозидов являются неселективными или предусматривают использование лабораторных животных (биологические тест-системы). При изучении фармакопейных методов идентификации сердечных гликозидов как в ЛРС, так и в составе лекарственных средств было показано, что актуальным остается использование химических методов (качественных реакций) и тонкослойной хроматографии. Установлено, что при количественной оценке ЛРС и экстракционных препаратов из него используются биологические или неселективные (спектрофотометрические) методы, хроматографические методы представлены только в фармакопейных статьях и монографиях на субстанции, содержащие индивидуальные сердечные гликозиды, и лекарственные препараты на их основе. Проведенный анализ стандартов качества и научных публикаций позволил выявить перспективные методы количественного определения сердечных гликозидов как в ЛРС, так и в фармацевтических субстанциях и лекарственных препаратах. Такими методами могут быть хроматографические методы.

Выводы

Выводы. Наиболее приемлемым методом для фармакопейного анализа является обращенно-фазовая высокоэффективная жидкостная хроматография (ВЭЖХ) со спектрофотометрическим детектированием. Разработка ВЭЖХ-методики количественного определения сердечных гликозидов позволит перейти от использования биологических или неселективных методов при анализе сердечных гликозидов к современному селективному методу анализа.

Scientific relevance

Scientific relevance. Cardiac glycosides have been used in medicine for over two centuries. Current studies suggest that biologically active substances from this group can be used to treat not only heart conditions but also viral infections, cancers, and other diseases. Therefore, quality control methods for cardiac glycosides are becoming increasingly relevant.

Aim

Aim. Based on a review of Russian and international quality standards, as well as up-to-date scientific data, this study aimed to identify promising methods for the identification and quantification of cardiac glycosides in herbal drugs and herbal medicinal products, as well as to evaluate the possibility of substituting physicochemical methods for biological methods.

Discussion

Discussion. The methods that are currently used to standardise cardiac glycosides are either not selective or require laboratory animals (biological test systems). According to a study of pharmacopoeial methods for the identification of cardiac glycosides in herbal drugs and herbal medicinal products, chemical identification tests and thin-layer chromatography continue to be relevant. Quantitative testing of herbal drugs and extracts uses biological and non-selective (spectrophotometry) methods, whereas chromatography is described only in general and individual monographs for herbal drug preparations containing individual cardiac glycosides and medicinal products containing these preparations. Upon analysing quality standards and scientific publications, the authors identified potentially promising methods for the quantification of cardiac glycosides in herbal drugs, herbal drug preparations, and herbal medicinal products, namely chromatographic methods.

Conclusions

Conclusions. Reverse-phase high-performance liquid cjromatography (HPLC) with spectrophotometric detection is the most suitable method for pharmacopoeial analysis. The development of an HPLC-based analytical procedure to determine the cardiac glycoside content will provide an opportunity to advance from biological or non-selective methods to more ethical and selective up-to-date techniques.

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The authors declare that there are no conflicts of interest present.