vedomostiregmedРегуляторные исследования и экспертиза лекарственных средствRegulatory Research and Medicine Evaluation3034-30623034-3453Federal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’)10.30895/1991-2919-2020-10-1-41-50vedomostiregmed-284Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESСравнительный анализ содержания тяжелых металлов и мышьяка в различных лекарственных формах растительных препаратов российского фармацевтического рынкаComparative Analysis of Heavy Metal and Arsenic Content in Various Herbal Dosage Forms Marketed in Russiahttps://orcid.org/0000-0001-9440-0950ЩукинВ. М.ShchukinV. M.

Щукин Виктор Михайлович.

Петровский бульвар, д. 8, стр. 2, Москва, 127051

Viktor M. Shchukin

8/2 Petrovsky Blvd, Moscow 127051

schukin@expmed.ruhttps://orcid.org/0000-0002-9133-0835КузьминаН. Е.Kuz’minaN. E.

Кузьмина Наталия Евгеньевна - доктор химических наук.

Петровский бульвар, д. 8, стр. 2, Москва, 127051

Natalia E. Kuz’mina - Dr. Sci. (Chem.).

8/2 Petrovsky Blvd, Moscow 127051

KuzminaN@expmed.ruhttps://orcid.org/0000-0002-2125-6174ШвецоваЮ. Н.ShvetsovaYu. N.

Швецова Юлия Николаевна.

Петровский бульвар, д. 8, стр. 2, Москва, 127051

Yulia N. Shvetsova.

8/2 Petrovsky Blvd, Moscow 127051

shvetsovajn@expmed.ruhttps://orcid.org/0000-0001-8752-5245ЛутцеваА. И.LuttsevaA. I.

Лутцева Анна Ивановна - кандидат фармацевтических наук.

Петровский бульвар, д. 8, стр. 2, Москва, 127051

Anna I. Luttseva - Cand. Sci. (Pharm.).

8/2 Petrovsky Blvd, Moscow 127051

Lutceva@expmed.ruФедеральное государственное бюджетное учреждение «Научный центр экспертизы средств медицинского применения» Министерства здравоохранения Российской ФедерацииРоссияScientific Centre for Expert Evaluation of Medicinal ProductsRussian Federation2020260220201014150Copyright © Щукин В.М., Кузьмина Н.Е., Швецова Ю.Н., Лутцева А.И., 20202020Щукин В.М., Кузьмина Н.Е., Швецова Ю.Н., Лутцева А.И.Shchukin V.M., Kuz’mina N.E., Shvetsova Y.N., Luttseva A.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vedomostincesmp.ru/jour/article/view/284

Введение в Государственную фармакопею Российской Федерации (ГФ РФ) требований по раздельному определению мышьяка, кадмия, ртути и свинца, а также современных способов пробоподготовки требует актуализации существующих норм по содержанию элементных токсикантов в лекарственном растительном сырье (ЛРС) и лекарственных растительных препаратах (ЛРП) на его основе.

Цель работы

Цель работы: анализ данных по содержанию элементных токсикантов, полученных при проведении экспертизы качества ЛРП (трав, сборов, экстрактов и настоек) с помощью современных методов анализа и пробоподготовки, а также сравнение полученных результатов с отечественными и зарубежными данными научной и специальной литературы.

Материалы и методы

Материалы и методы: собственные экспериментальные данные по содержанию нормируемых тяжелых металлов и мышьяка в различных лекарственных формах лекарственных растительных препаратов, полученные методом масс-спектрометрии с индуктивно-связанной плазмой с использованием в качестве пробоподготовки разложения в закрытых сосудах, сравнивались с данными других авторов.

Результаты

Результаты: установлено, что содержание свинца, кадмия и ртути во всех исследованных образцах не превышает установленных в ГФ РФ норм и соответствует проанализированным данным литературы. Содержание мышьяка в ряде ЛРП превышает российские нормативы, но удовлетворяет менее жестким нормам Европейской фармакопеи и Фармакопеи США для ЛРС. Изучена зависимость содержания элементных токсикантов от места сбора и морфологических частей растений. Отмечена особенность накопления отдельных элементов различными видами лекарственных растений. Определено содержание нормируемых элементов в экстрактах и настойках. Сделано предположение, что различие содержания элементных токсикантов в отечественных и зарубежных требованиях связано со способом получения экспериментальных данных, которые являются основой нормирования.

Выводы

Выводы: проведенные исследования позволяют сделать выводы о пригодности существующих норм содержания элементных токсикантов в ЛРП. Обоснована необходимость пересмотра существующих требований по содержанию мышьяка в ЛРП.

The inclusion of requirements for independent determination of arsenic, cadmium, mercury, and lead, and the current sample preparation techniques into the State Pharmacopoeia of the Russian Federation (Ph. Rus.) requires the revision of the existing limits for elemental toxicants in herbal substances and herbal medicinal products produced from them.

The aim of the study was to analyse the data on elemental toxicant content obtained during quality control of herbal substances (herbs, medicinal herb mixtures, extracts, and tinctures) using current test methods and sample preparation techniques, and to compare the obtained results with the Russian and foreign scientific and specialist literature.

Materials and methods

Materials and methods: the internal data on the content of critical heavy metals and arsenic in different dosage forms of herbal medicinal products, which were obtained by inductively coupled plasma mass spectrometry after sample preparation by decomposition in closed vessels, were compared with literature data. Results: it was demonstrated that the content of lead, cadmium, and mercury in all the test samples did not exceed the Ph. Rus. limits and was consistent with the analysed literature. The arsenic content in some herbal medicinal products was higher than the established Ph. Rus. limits, but complied with the less stringent Ph. Eur. and USP requirements for herbal substances. The authors investigated the link between the content of elemental toxicants and the place of collection and the part of the plant being tested. It was shown that different types of medicinal plants had a tendency to accumulate particular elements. The authors determined the content of the elements to be controlled in extracts and tinctures. The differences in the Russian and foreign requirements for the content of elemental toxicants may be attributed to the method of obtaining experimental data that form the basis for the setting of limits.

Conclusions

Conclusions: the results of the study confirm the validity of the existing limits for elemental toxicants in herbal medicinal products. The authors demonstrated the need to revise the existing limits for arsenic in herbal medicinal products.

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The authors declare that there are no conflicts of interest present.